Please note that this is a draft schedule and will inevitably change to some extent.
1 | 8/28 | Starter Cultures
In Class
- Welcome
- Lecture: What is Biofabrication?
- Website Overview
- Syllabus
Video: Suzanne Lee- Introduction to the Bio Lab
- EHS
- Communication
- Lab Work: G.hansenii Starter Culture for growing Bacterial Cellulose
- Work in teams of two students
- Make HS Media
- Each team will make 1L of media
- Inoculate 10 mL cultures
- 2 cultures per student
- one negative control per team
Homework
- Website
- if your Google profile picture is not a recognizable image of yourself, please change it
- log in to the class website, using the “continue with google” option
- confirm that your photo shows up on the the class People page
- if needed, you can make changes to your “display name” on your profile page
- Lab
- finish making media (if needed)
- check on cultures for cellulose growth -> pellicle formation -> thickness of 2mm+
- check on Negative Control
- Reading
- Prepare for next week’s lab session:
- Review units of measurement for the bio lab
- Watch pipetting tutorial videos (~7 minute)
2 | 9/04 | Labor Day
3 | 9/11 | Culture Growth
In Class
- Discussion
- Presentation
- Video – Suzanne Lee: Grow your own clothes
- Lecture – Biofabrication with Bacterial Cellulose
- Lab
- scale up G. hansenii cultures to 200mL in 500mL Erlenmeyer flasks
- discuss next scale up to 1-5L
- look at cells and cellulose under the microscope
- serial dilution lab exercise to determine cell culture density
Homework
- Lab
- Serial Dilution
- check on plates
if you cannot make these times, ask a classmate to photograph your plates
once you have readable plates, no further check-in are required
additional dates will be scheduled if needed- Tues 3:00-4:00
- Wed 2:30-3:30
- once plates are readable
- count the colonies on the plates
- photograph the plates
- log the data in the spreadsheet
- check on plates
- G. hansenii Cultures
- begin planning your bacterial cellulose project
- make HS media
- determine volume needed, based on the desired size pellicle and needed vessel
- make media and autoclave it
- consolidate your media as soon as possible to free up bottles
- Serial Dilution
- Reading
- Prepare for next week’s lab session:
- Review units of measurement for the bio lab
- Watch pipetting tutorial videos (~7 minute)
4 | 9/18 | Scaling Up
In Class
- Lab
- examine serial dilution exercise results
- review plates
- spreadsheet data
- discuss results
- examine 200mL G. hansenii cultures
- scale up G. hansenii cultures to 1-5L
- look at G. hansenii and cellulose under the microscope
- examine serial dilution exercise results
- Lecture
- Discussion
- Synthetic Biology: A Primer, Ch 1
Homework
- Read (printouts are in the cubbies just outside of the lab)
- CRISPR lab guide pgs 1-21
- Do worksheet exercises in Part I
- Synthetic Aesthetics Ch1 “Synthetic Biology: What It Is and Why It Matters” by Alistair Elfick and Drew Endy
5 | 9/25 | CRISPR Part I – Classroom
In Class
- Review CRISPR text and worksheet
- Prepare for CRISPR lab
- Discuss Synthetic Aesthetics Ch1 “Synthetic Biology: What It Is and Why It Matters” by Alistair Elfick and Drew Endy
- Introduce 2D Bioprinting Project
- Lecture: Artists using synthetic biology
- check on G. hansenii cultures
Homework
- review CRISPR Lab
- begin planning 2D Bioprinting Project
6 | 10/02 | CRISPR Part II – Lab
In Class
- CRISPR lab
- check on G. hansenii cultures
- discuss 2D Bioprinting Plans
Homework
- check on CRISPR plates
- Tues 3:00-4:00
- Wed 4:30-5:30
- begin planning and preparing for 2D Bioprinting Project
- gather tools
- create stencils
7 | 10/09 | Bioprinting Work
8 | 10/16 | Fall Break
9 | 10/23 | Critique Bioprinting Project
10 | 10/30 | Cellulose Harvest
11 | 11/06 | Cellulose Conditioning
12 | 11/13 | Critique Bacterial Cellulose Project
13 | 11/20 | Mycelium Inoculation
14 | 11/27 | MycoCasting
15 | 12/04 | Growing Networks
16 | 12/11 | Critique Mycelium Project