Please note that this is a draft schedule and will inevitably change to some extent.
weeks 1-7 – genetic engineering and bioprinting
weeks 8-13 – biomaterials
weeks 14-16 – speculative biotech

1   |   8/30   |  An Introduction to Biofabrication


In Class

Homework

2  |   9/6  |   Intro to Synthetic Biology

In Class

  • Discuss readings
  • Lab I – cell culture density analysis
    • Lab safety
    • optical density
    • serial dilutions
    • Pipetting
    • Positive and Negative control
    • lab notes
    • set times for lab access
  • overview of synthetic biology
    • DNA structure
    • genetic parts
    • engineered standardized parts
    • restriction enzymes
    • biobricks assembly
    • CRISPR
  • Introduce Lab II

Homework

3  |   9/13  |   Lab II – Genetic Engineering with CRISPR+Cas9

In Class

  • Review results of Lab I
  • Review readings
  • Technical overview of genetic engineering
    • with restriction enzymes
    • with CRISPR+Cas9
  • Lab II – CRISPR+Cas9
    • paper model (moved to homework)
    • lab exercise

Homework

  • complete questions in lab protocol handout (“Activity 2”)
  • complete paper CRISPR worksheet
  • gather material and ideas for 2D Bioprinting experimentation

4  |   9/20  |  2D Bioprinting Experimentation

In Class

  • review CRISPR lab results and discuss
  • explore different bacterial “printing” processes

Homework

  • review results of experiments
  • plan for project I
  • prepare materials, tools, and/or files for in-class work

5  |   9/27  |  Bioprinting Work Day

In Class

  • Lecture: BioArt Gen I
  • Print with genetically modified bacteria
  • Discuss bioprinting project ideas

Homework

  • DO: complete bacterial prints
    • document process
    • photograph results

6  |  10/4  |  Critique

In Class

  • critique
  • document

Homework


7  |   10/11  |  Biomaterials I

In Class

  • introduce biomaterials

Homework

  • prep media for biomaterials

8  |   10/18  |  Fall Break

8  |   10/25  |  Shifting Perspectives

In Class

  • alternative to the engineering perspective in biology
  • Lab: Biomaterials
    • inoculate media
    • discuss monitoring
    • discuss post-processing

Homework

  • tend biomaterial cultures
  • monitor for contamination
    • log all observations
    • report any contamination
      • properly dispose of contamination
      • identify possible cause
      • re-start culture
  • material experiments with provided samples
    • record all experiments with detailed lab notes and photo documentation
    • upload documentation
  • Resisting Biopolitics assignment
    • select an essay from Resisting Biopolitics to read
    • write a 1-3 pg report on it
    • prepare to give a 5 minute summary in class

10  |   11/1  |  Biomaterial Growth

In Class

  • no class

Homework

  • tend biomaterial cultures
  • monitor for contamination
    • log all observations
    • report any contamination
      • properly dispose of contamination
      • identify possible cause
      • re-start culture
  • material experiments with provided samples
    • record all experiments with detailed lab notes and photo documentation
    • upload documentation
  • complete Resisting Biopolitics assignment

11  |   11/8  |  Biomaterials Harvest and Process

In Class

  • discuss Biopolitics essays
  • review material experiment
  • discuss biomaterials project proposals
  • harvest materials
  • begin post-processing

Homework

  • work on biomaterials project

12  |   11/15  | Biomaterials Work Day

In Class

  • review biomaterials progress
  • work time
  • lecture

Homework

  • complete on Biomaterials Project
  • continue documentation

13  |   11/23  | Critique

In Class

  • critique biomaterials project
  • introduce speculative project

Homework

14  |   12/6  | Complex Collaborations

In Class

  • Discuss Haraway reading
  • Lecture: The art of living with
  • Sympoeitic exercises

Homework

  • work on project
  • varied readings

15  |   12/13  | Work Day

In Class

  • work on project

Homework

  • complete project

16  |   12/20  | Critique

In Class

  • critique