Please note that this is a draft schedule and will inevitably change to some extent.
weeks 1-7 – genetic engineering and bioprinting
weeks 8-13 – biomaterials
weeks 14-16 – speculative biotech

1   |   8/26   |  An Introduction to Biofabrication


In Class

Homework

2  |   9/2  |   Labor Day

3  |   9/9  |   Intro to Synthetic Biology

In Class

  • BioArt – Gen1
  • how nature makes stuff
    • review readings
    • breaking down the central dogma of molecular biology
  • Lab I – cell culture density analysis
    • Lab safety
    • optical density
    • serial dilutions
    • Pipetting
    • Positive and Negative control
    • lab notes
  • quiz review
  • overview of synthetic biology
    • standardized part
    • restriction enzymes
    • biobricks assembly
  • Overview of Lab II
    • Digestion
    • Ligation

Homework

4  |   9/16  |  Lab I – Synthetic Biology

In Class

  • Review Lab I
  • Lab II: Synthetic Biology
    • Digestion
    • Ligation
    • Transformation
    • Recovery
    • Plate cells

Homework

  • DO: Mini-lab sessions
    • Tuesday 3:00-4:00 check on growth
      • examine plates for colonies
      • use UV light to look for fluorescent protein expression
      • record observations in spreadsheet
      • if growth is not strong or evident at all, give them another day in the incubator
    • Wednesday 3:00-4:00 repeat
  • Sunday 11:00-4:00 culture cells in liquid media
    • examine plates for colonies
    • use UV light to look for fluorescent protein expression
    • select a good looking colony
    • inoculate LB media with colony
      • touch the colony with 10uL pippette tip
      • place the tip in 10mL of liquid LB media in a 16mm x 150mm test tube
      • pipette up and down several times
    • cap tube loosely, label it,  and incubate cells at 37C overnight in shaker
  • DO: Prepare materials for bacterial printing- do one or more of the following:
    • Stencil
    • prepare files for syringe-based bioprinting
      • create a 2D drawing in Rhino
      • we will convert this to Gcode using the Grasshopper plugin, Xylinus
    • devise some other method of applying cells to your agar plate
      • paint brushes?
      • other tools?

5  |   9/23  |  2D Bioprinting

In Class

  • Lecture: Art and Biopolitics
  • Print with genetically modified bacteria
  • Discuss bioprinting project ideas

Homework

6  |  9/30  |  Bioprinting Work Day

In Class

  • discuss “Resisting Biopolitics”
  • introduce biopolitics assignment
  • Lab work time

Homework

7  |   10/7  |  Critique

In Class

  • critique work
  • introduce biomaterials

Homework

8  |   10/14  |  Shifting Perspectives

In Class

Homework

  • tend biomaterial cultures
  • monitor for contamination
    • log all observations
    • report any contamination
      • properly dispose of contamination
      • identify possible cause
      • re-start culture
  • material experiments with provided samples
    • record all experiments with detailed lab notes and photo documentation
    • upload documentation
  • Resisting Biopolitics assignment
    • select an essay from Resisting Biopolitics to read
    • write a 1-3 pg report on it
    • prepare to give a 5 minute summary in class

9  |   10/21  |  Fall Break

10  |   10/28  |  Biomaterial Growth

In Class

  • no class

Homework

  • tend biomaterial cultures
  • monitor for contamination
    • log all observations
    • report any contamination
      • properly dispose of contamination
      • identify possible cause
      • re-start culture
  • material experiments with provided samples
    • record all experiments with detailed lab notes and photo documentation
    • upload documentation
  • complete Resisting Biopolitics assignment

11  |   11/4  |  Biomaterials Harvest and Process

In Class

  • discuss Biopolitics essays
  • review material experiment
  • discuss biomaterials project proposals
  • harvest materials
  • begin post-processing

Homework

  • work on biomaterials project

12  |   11/4  | Biomaterials Work Day

In Class

  • review biomaterials progress
  • work time
  • lecture

Homework

  • complete on Biomaterials Project
  • continue documentation

13  |   11/6  | Critique

In Class

  • critique biomaterials project
  • introduce speculative project

Homework

  • complete documentation
  • plan speculative project
  • read Donna Harroway

14  |   11/25  | Complex Collaborations

In Class

  • strange perspectives
    • microbiomes
    • subject/environment (holobiont)
    • contextual toxicity
    • interdependence

Homework

  • work on project
  • varied readings

15  |   12/2  | Work Day

In Class

  • work on project

Homework

  • complete project

16  |   12/9  | Critique

In Class

  • critiqu